At FDU: BIOL3225/3226 General Microbiology

Instructor:
Dr. Alice Benzecry

e-mail: benzecry@fdu.edu
Office: Dickinson Hall, Room 4416
Phone # 692-2385

FALL 2008
Office Hours: Tuesday 9:00 to 11:30AM
or by appointment

BIOL3225/3226 General Microbiology

Syllabi

4 Credits
Lecture (2 hours) Laboratory (4 hours)
Prerequisites: Biological Sciences I & II and Organic Chemistry I & II.

***All students are expected to have completed their Chemistry requirements prior to taking this course. I expect all students to be familiar with all chemical concepts and pathways.

Course Description:
Introduction to microorganisms structure, biochemistry, genetics and physiology, their interactions with animals and other organisms. Laboratory deals with isolation and identification of common pathogenic and nonpathogenic organisms utilizing staining, culturing, biochemical analysis and microscopic inspection.

Requirements:
Lecture textbook: MICROBIOLOGY. 8th. Edition, . By Prescott, Harley & Klein. McGraw-Hill
Laboratory manual: Laboratory Exercises in Microbiology. 8th Edition; By Harley & Prescott. McGraw-Hill.
Photographic Atlas: Microbiology - A photographic Atlas for the laboratory By K. Alexander and D. Strete. BenjaminCummings.
Lab Notebook: small composition notebook to be used exclusively for this lab
Lab Coat: You must obtain a lab coat during the first week of class. Student without lab coat will not be admitted into the laboratory.

BIOL. 3225 GENERAL MICROBIOLOGY - LECTURE OUTLINE

Lecture Period TOPIC Assigned Text Reading

1 - 3      Scope and History of Microbiology Introduction to Microbiology: Microorganisms, Branches of Microbiology, Future of Microbiology and History of Microbiology Chapter 1
4      Diversity of the microbial world Taxonomy Chapter 19
5 - 6      Virus Selective reading from chapters16, 17, 18
7      Eucaryotic Cell Structures and Functions Domain Eukarya - Fungi., Algae,.Protozoa, and.Helminthes Chapters 4 and selective reading from chapters 25, 26, 27
8-9      Overview of Prokaryotic cell Structure: Prokaryotic Cell Membrane, Prokaryotic Cytoplasmic Matrix, Prokaryotic Cell Wall, External Components, Bacteria Endospore, and Chemotaxis Domains Archaea and Bacteria Chapters 3, and selective readings of chapter 20
10      EXAM 1
11 - 13      Microbial Nutrition and growth: Nutritional requirements, measurement of microbial growth, Closed Culture/Continuous Culture, Growth Curve, Growth in Natural environment, Influence of environmental factors on growth Chapters 5 & 6
14 - 16      Use of Physical and Chemical Agents as population Controlling Agents. Chapter 7
17-20      Microbial Metabolism - Catabolism: Respiration, Fermentation, Photosynthesis Chapters 8 & 9
21      EXAM 2
22-23       Anabolism / Biosynthesis Chapters 9 & 10
24 - 26      Microbial Genetics: Nucleic Acid Structure – DNA / RNA, Gene Structure and Genetic Code, Prokaryotic, Protein Synthesis. Chapter 11
27      Prokaryotic DNA Replication: - Conjugation Bi-directional replication & Rolling circle Replication Chapter 12
28      Alterations in genetic information of the bacterial Cell: Genotype / phenotype, Mutations, Transformation, and Transduction Chapter 13
     FINAL EXAM

BIOL. 3226 GENERAL MICROBIOLOGY LABORATORY

LABORATORY SCHEDULE

Period      TOPIC      Assigned Reading

1      Introduction Laboratory safety and Protocol. Part I: Microscopy: Overview of Different microscopes Chapter 2 of text book Ex.# 3, 4, & 5
2      Use of Bright Field Microscope and calibration Ex.# 1
3-6      Part 10: Survey of selected Eukaryotic and Prokaryotic organisms in the environment. Fungi, Algae, protozoan, & helminthes Ex. # 53, 59, 60, and Handout
7      Part 2: Aseptic techniques and Specimen preparation: Negative & Simple Staining Ex.# 6 7 & 8
8      Gram Stain and Acid-fast Ex.# 8 & 9
9      Endospore, Capsule and Flagella Staining Ex.# 10, 11,12
10-11      Part 3: Basic Laboratory and Culture Techniques: Culture Media preparation, Culture transfer and isolation of pure cultures Cultivation of Anaerobic Bacteria, Streak plate, Spread, and Pour-plate Chapter 5 of text book Ex.# 13, 14, 15, 16 & 17,
12      Differential and Selective Media 18 + Hand-out
13      Results and Review for Mid-term
     MIDTERM
14      Determination of Bacterial # Ex.# 19 Chapter 6 of text book
15      Part4 : Biochemical Activities: Carbohydrate Fermentation (Durham tube) Triple Sugar Iron Agar test Ex.# 20 & 21
16      Starch, Lipid, and Protein hydrolysis Ex.# 22, 23, & 26
17      Gelatin and Litmus Milk Reaction Ex.# 27 + Hand-out
18 Catalase, Oxidase, DNase, Urease, and Nitrate Reduction Tests Ex.# 28, 29, 30, 31 & 34
19      Hydrogen Sulfide Production and Motility IMViC Tests and Enterotube II Ex.# 24, 25 & 36
20      Part 7: Environmental Factors: Temperature and pH Chapter 7 of text book Ex.# 39 & 40
21      Osmotic Pressure & Antibiotics (Kirby-Bauer Method) Ex.# 41 & 43
22      Ultraviolet Radiation Hand-out
23      Results and Review
24-28      UNKNOWN ID All lab material must be completed by the last day of classes.

Links of interest:

Role of Microorganisms
Sciences education
Center for Disease Control

Email address: benzecry@fdu.edu

Copyright © 2006, BIOL3225/3226 General Microbiology, except images copyright Fairleigh Dickinson University, used with permission.
This page originally created with FDU Pagetoaster. Click to see how'd they do that?